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il 1ra  (Novus Biologicals)


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    Novus Biologicals il 1ra
    H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor <t>(IL‐1Ra),</t> and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.
    Il 1ra, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nbp1+32568/IL-1ra%2FIL-1F3%2FIL1RN+Antibody/pmc12893307-351-34-36
    Average 93 stars, based on 3 article reviews
    il 1ra - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Mannose Oligosaccharide‐Conjugated In Situ Pore‐Forming Injectable Hydrogels for Rheumatoid Arthritis Treatment by Reprogramming Macrophage Extracellular Vesicles"

    Article Title: Mannose Oligosaccharide‐Conjugated In Situ Pore‐Forming Injectable Hydrogels for Rheumatoid Arthritis Treatment by Reprogramming Macrophage Extracellular Vesicles

    Journal: Small Methods

    doi: 10.1002/smtd.202500605

    H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor (IL‐1Ra), and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.
    Figure Legend Snippet: H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor (IL‐1Ra), and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.

    Techniques Used: Comparison, Flow Cytometry, Cell Culture, Western Blot, Two Tailed Test

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    Article Snippet: The following primary antisera were used, for each of which the host species is denoted in square brackets: insulin (A0564 [guinea pig], 1:4,000; Dako, Carpenteria, CA), IL-1β (49-4960 [rabbit], 1:500; ProSci Incorporated, Poway, CA; and ab2105 [rabbit], 1:1,000; Abcam, Cambridge, MA), IL-1Ra (AF-280-NA [goat], 1:500; R&D Systems, Minneapolis, MN; and NBP1-32568 [rabbit], 2 μg/mL; Novus Biologicals, Littleton, CO), CD68 (Clone 514H12 [mouse], 0.34 μg/mL; Leica Biosystems/Novacastra), glucagon (EP3070 [rabbit], 1:10,000; Epitomics, Burlingame, CA), somatostatin (SC-7819 [goat], 1:200; Santa Cruz Biotechnology, Santa Cruz, CA), and pancreatic polypeptide (PP) (NB100-1793 [goat], 1:250; Novus).



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    il 1ra  (Novus Biologicals)
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    Novus Biologicals il 1ra
    H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor <t>(IL‐1Ra),</t> and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.
    Il 1ra, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nbp1+32568/IL-1ra%2FIL-1F3%2FIL1RN+Antibody/pmc12893307-351-34-36
    Average 93 stars, based on 1 article reviews
    il 1ra - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    nbp1 32568  (Novus Biologicals)
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    H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor <t>(IL‐1Ra),</t> and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.
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    H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor (IL‐1Ra), and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.

    Journal: Small Methods

    Article Title: Mannose Oligosaccharide‐Conjugated In Situ Pore‐Forming Injectable Hydrogels for Rheumatoid Arthritis Treatment by Reprogramming Macrophage Extracellular Vesicles

    doi: 10.1002/smtd.202500605

    Figure Lengend Snippet: H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor (IL‐1Ra), and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.

    Article Snippet: The membrane pieces were probed overnight at 4 °C with primary antibodies against GAPDH (#2118, Cell Signaling Technology, CST, Danvers, MA, USA), CD206 (ab64693, Abcam), CD44 (ab157107, Abcam), IFN‐γ (ab198801, Abcam), TNF‐α (ab307164, Abcam), IL‐1Ra (NBP1‐32568, Novus Biologicals, Littleton, CO, USA) and Arg‐1 (#93668, CST).

    Techniques: Comparison, Flow Cytometry, Cell Culture, Western Blot, Two Tailed Test